Transglycosylation of Rebaudioside А by β-fructofuranosidase
نویسندگان
چکیده
منابع مشابه
Production of b-Fructofuranosidase by Arthrobacter sp. and Its Application in the Modification of Stevioside and Rebaudioside A
Arthrobacter sp. 10137 has been used to produce b-fructofuranosidase (FFase). Sucrose and corn steep powder in an optimized ratio of 10:1 were the best carbon and nitrogen sources for enzyme production in a shake flask. The maximum FFase activity was 26.69 U/mL after 22.5 h in batch culture, and the crude FFase, obtained by ultrafiltration and (NH4)2SO4 fractionation, was purified about 7-fold ...
متن کاملCharacterization of recombinant β-fructofuranosidase from Bifidobacterium adolescentis G1
BACKGROUND We have previously reported on purification and characterization of beta-fructofuranosidase (beta-FFase) from Bifidobacterium adolescentis G1. This enzyme showed high activity of hydrolysis on fructo-oligosaccharides with a low degree of polymerization. Recently, genome sequences of B. longum NCC2705 and B. adolescentis ATCC 15703 were determined, and cscA gene in the both genome seq...
متن کاملIdentification of sucA, Encoding β-Fructofuranosidase, in Rhizopus microsporus
Rhizopus microsporus NBRC 32995 was found to hydrolyze fructooligosaccharides (FOS), as well as sucrose, almost completely into monosaccharides through the production of sufficient amounts of organic acids, indicating that the complete hydrolysis of FOS was caused by the secretion of β-fructofuranosidase from fungal cells. Thus, the sucA gene, encoding a β-fructofuranosidase, was amplified by d...
متن کاملBioconversion of rebaudioside I from rebaudioside A.
To supply the increasing demand of natural high potency sweeteners to reduce the calories in food and beverages, we have looked to steviol glycosides. In this work we report the bioconversion of rebaudioside A to rebaudioside I using a glucosyltransferase enzyme. This bioconversion reaction adds one sugar unit with a 1→3 linkage. We utilized 1D and 2D NMR spectroscopy (1H, 13C, COSY, HSQC-DEPT,...
متن کاملMutagenesis of Aspergillus Oryzae IPT-301 to improve the production of β-Fructofuranosidase
Aspergillus oryzae IPT-301, previously reported as a β-fructofuranosidase producing microorganism, was successfully mutated using UV irradiation at 253.7 nm followed by the screening of survivors resistant to certain stress conditions. Strains were first subjected to the β-fructofuranosidase activity assay using a portion from the colony grown in Petri dish as the enzyme source. Seven mutants w...
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ژورنال
عنوان ژورنال: Health, Food & Biotechnology
سال: 2019
ISSN: 2712-7648
DOI: 10.36107/hfb.2019.i1.s82